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Expression data from H1299 human non-small cell lung carcinoma cell lines stably expressing CHES1 compared with H1299 infected with an empty vector

ABSTRACT: The expression of the forkhead transcription factor CHES1, also known as FOXN3, is reduced in many types of cancers. In vitro, CHES1 expression suppresses cell proliferation in tumor cell lines but not in normal cells. Conversely shRNA-mediated depletion of CHES1 increases tumor cell proliferation. We used microarrays to characterize the gene expression changes induced by CHES1 in H1299 cancer cells H1299 cells were infected with a retroviral vector that expresses CHES1 or an empty vector. After 5 days of infection, RNA was extracted from three independent replicas of each condition. Total RNA was send to Genome Quebec service for hybridization with Affymetrix microarrays.

ORGANISM(S): Homo sapiens

SUBMITTER: Vernier Mathieu

PROVIDER: S-ECPF-GEOD-49422 | biostudies-other |

REPOSITORIES: biostudies-other

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Expression data from H1299 human non-small cell lung carcinoma cell lines stably expressing CHES1 compared with H1299 infected with an empty vector

2013-08-01 | GSE49422 | GEO

Expression data from H1299 human non-small cell lung carcinoma cell lines stably expressing CHES1 compared with H1299 infected with an empty vector

Project description:The expression of the forkhead transcription factor CHES1, also known as FOXN3, is reduced in many types of cancers. In vitro, CHES1 expression suppresses cell proliferation in tumor cell lines but not in normal cells. Conversely shRNA-mediated depletion of CHES1 increases tumor cell proliferation. We used microarrays to characterize the gene expression changes induced by CHES1 in H1299 cancer cells H1299 cells were infected with a retroviral vector that expresses CHES1 or an empty vector. After 5 days of infection, RNA was extracted from three independent replicas of each condition. Total RNA was send to Genome Quebec service for hybridization with Affymetrix microarrays.

2013-08-01 | E-GEOD-49422 | biostudies-arrayexpress

Expression data of NCI-H441 cells stably expressing hsa-mir-365-2 vs empty vector

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RNA-seq of H1299 cells stably transfected with empty vector or the p53 mutant R273H.

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2017-04-16 | E-MTAB-5653 | biostudies-arrayexpress

RNA-seq of MCF10A cells stably expressing empty vector, GFP, miR-105, or MYC

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Expression data for nasopharyngeal carcinoma cell line 5-8F stably expressing either LTF gene or empty vector.

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Expression data of NCI-H441 cells stably expressing hsa-mir-365-2 vs empty vector

Project description:Hsa-mir-365-2 is one of the two precursors that give rise to miR-365. We discovered that miR-365 directly regulates a lung cancer and developmental gene termed thyroid transcription factor 1 (TTF-1 or NKX2-1). Hsa-mir-365-2 was transfected into NCI-H441 cells via retrovirus-mediated gene transfer. Arrays hybridized: Affymetrix GeneChip Human Gene 1.0 ST Array.

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Expression data for nasopharyngeal carcinoma cell line 5-8F stably expressing either LTF gene or empty vector.

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2013-12-26 | E-GEOD-36972 | biostudies-arrayexpress

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