Transcription profiling of human breast epithelia cells after 17-beta-estradiol induced malignant cell transformation

ABSTRACT: The estrogen-dependence of breast cancer has long been recognized, however, the role of 17β-estradiol (E2) in cancer initiation was not known until we demonstrated that it induces complete neoplastic transformation of the human breast epithelial cells MCF-10F. E2-treatment of MCF-10F cells progressively induced high colony efficiency and loss of ductulogenesis in early transformed (trMCF) cells and invasiveness in Matrigel invasion chambers. The cells that; crossed the chamber membrane were collected and identified as bsMCF, and their subclones designated bcMCF, and the cells harvested from carcinoma formation in SCID mice designated caMCF. These phenotypes correlated with gene dysregulation during the progression of the ; transformation. The highest number of dysregulated genes was observ

ORGANISM(S): Homo sapiens

SUBMITTER: Huang Y 

PROVIDER: S-ECPF-GEOD-5116 | biostudies-other | 2007 Dec

REPOSITORIES: biostudies-other