Genome-wide activity of unliganded Estrogen Receptor alpha in breast cancer cells [RNA-Seq]

ABSTRACT: ERα is essential for the anti-proliferative response of breast cancer cells not only to estrogen antagonists, but also to estrogen withdrawal by means of aromatase inhibitors. We explored here one of the simplest explanation for this, consisting in the possibility that ERα may have a wide genomic function in absence of ligands. The genomic binding of ERα in the complete absence of estrogen was then studied using hormone-dependent MCF7 cells, by chromatin immunoprecipitation sequencing. From these data, 4.2K highly significant binding events were identified, which were further confirmed by comparing binding events in cells expressing ERα to cells silenced for ERα. Apo-ERα binding sites were distributed close to genes with functions associated to cell growth and epithelial maintenance and sh

ORGANISM(S): Homo sapiens

SUBMITTER: Ferrero Giulio 

PROVIDER: S-ECPF-GEOD-53532 | biostudies-other |

REPOSITORIES: biostudies-other