Inflammation-induced chemokine expression in uveal melanoma cell lines stimulates monocyte chemotaxis

ABSTRACT: Purpose: Uveal melanoma (UM) is the most common primary intraocular tumor in adults and the presence of infiltrating leucocytes is associated with a poor prognosis. Little is known how infiltrating leucocytes influence the tumor cells. The purpose of this study was to investigate the effect of activated T cells on the expression of chemotactic cytokines in UM cells. Furthermore, we examined the ability of stimulated UM cells to attract monocytes. Methods: We used an in vitro co-culture system in which UM cell lines and T cells were cultured together, but separated by a membrane. UM gene expression was quantified using a microarray. Protein expression in the supernatant was quantified with ELISA or cytometric bead array. For the monocyte migration assay, a trans-well plate was used. Resul

ORGANISM(S): Homo sapiens

SUBMITTER: Jehs Tina 

PROVIDER: S-ECPF-GEOD-55983 | biostudies-other |

REPOSITORIES: biostudies-other