Discovery of biomarkers predictive of GSI response in triple negative breast cancer and adenoid cystic carcinoma

ABSTRACT: Next generation sequencing was used to identify Notch mutations in a large collection of diverse solid tumors. NOTCH1 and NOTCH2 rearrangements leading to constitutive receptor activation were confined to triple negative breast cancers (TNBC, 6 of 66 tumors). TNBC cell lines with NOTCH1 rearrangements associated with high levels of activated NOTCH1 (N1-ICD) were sensitive to the gamma-secretase inhibitor (GSI) MRK-003, both alone and in combination with pacitaxel, in vitro and in vivo, whereas cell lines with NOTCH2 rearrangements were resistant to GSI. Immunohistochemical staining of N1-ICD in TNBC xenografts correlated with responsiveness, and expression levels of the direct Notch target gene HES4 correlated with outcome in TNBC patients. Activating NOTCH1 point mutations were also ident

ORGANISM(S): Homo sapiens

SUBMITTER: Stoeck A 

PROVIDER: S-ECPF-GEOD-59810 | biostudies-other | 2014 Oct

REPOSITORIES: biostudies-other