Comparing the effects of MITF on transcriptional regulation to the TEN1-ICD

ABSTRACT: We identified histidine triad nucleotide binding protein 1 (HINT1) as a human teneurin-1 ICD interaction partner in a yeast-2 hybrid screen. This interaction was confirmed in human cells, where HINT1 is known to inhibit the transcription of target genes by directly binding to transcription factors at the promoter. In a whole transcriptome analysis of BS149 glioblastoma cells overexpressing the teneurin-1 ICD, several microphthalmia-associated transcription factor (MITF) target genes were found to be up-regulated. Interestingly, MITF is one of the transcription factors inhibited by HINT1. Thus, we directly compare the transcriptomes of MITF versus TEN1-ICD overexpressing BS149 cells in this study, in order to reveal any co-regulated genes. For the whole transcriptome analysis of MITF, cells

ORGANISM(S): Homo sapiens

SUBMITTER: Schöler J 

PROVIDER: S-ECPF-GEOD-61705 | biostudies-other | 2015 Mar

REPOSITORIES: biostudies-other