Transcription profiling by array of human MCF7 cells after treatment with epidermal growth factor or heregulin

ABSTRACT: ErbB receptor ligands, epidermal growth factor (EGF) and heregulin (HRG), induce dose-dependent transient and sustained intracellular signaling, proliferation and differentiation of MCF-7 breast cancer cells, respectively. In an effort to delineate the ligand-specific cell determination mechanism, we investigated time-course gene expressions induced by EGF and HRG that induce distinct cellular phenotypes in MCF-7 cells. To analyze the effects of ligand dosage and time for the gene expression independently, we developed a statistical method for decomposing the expression profiles into the two effects. Our results indicated that signal transduction pathways devotedly convey quantitative properties of the dose-dependent activation of ErbB receptor to early transcription. The results also impl

ORGANISM(S): Homo sapiens

SUBMITTER: Nagashima T 

PROVIDER: S-ECPF-GEOD-6462 | biostudies-other | 2007 Feb

REPOSITORIES: biostudies-other