Transcription profiling by array of human breast cancer mammospheres and breast tumors

ABSTRACT: Tumorigenic breast cancer cells characterized by CD44 expression and low or undetectable CD24 levels (CD44+/CD24-/low) may be resistant to chemotherapy and therefore responsible for cancer relapse. Paired breast cancer core biopsies before and after neoadjuvant chemotherapy or lapatinib were obtained and as single cell suspensions stained using antibodies against CD24, CD44, and lineage markers, and then analyzed by flow cytometry. Mammosphere (MS) formation in culture was compared before and after treatment. Global gene expression differences between cancer cells bearing CD44+/CD24-/low cells and all other sorted cells, and between cancer MS and the primary bulk invasive cancers were analyzed. We report that CD44+/CD24-/low tumorigenic breast cancer cells were intrinsically chemoresistant

ORGANISM(S): Homo sapiens

SUBMITTER: Creighton CJ 

PROVIDER: S-ECPF-GEOD-7515 | biostudies-other | 2009 Aug

REPOSITORIES: biostudies-other