Transcription profiling of human pancreatic carcinoma growing in the pancreas of nude mice

ABSTRACT: Using Affymetrix HG-U133-Plus 2.0 array and Laser Capture Microdissection techniques, we determined whether growth in different zones of the same tumor affected expression of genes by human pancreatic cancer cells. Human L3.6pl pancreatic cancer cells were implanted into the pancreas of nude mice. Gene expression patterns in tumor cells within the central and peripheral zones were compared and statistical differences were determined for 1222 genes. Bioinformatic functional prediction analysis revealed that 346 upregulated genes in the peripheral zone were related to cytoskeleton organization and biogenesis, cell cycle, cell adhesion, cell motility, DNA replication, localization, integrin-mediated signaling pathway, development, morphogenesis, and IkB kinase/NF-kB cascade; and 876 upregu

ORGANISM(S): Homo sapiens

SUBMITTER: Nakamura T 

PROVIDER: S-ECPF-GEOD-7824 | biostudies-other | 2007 Aug

REPOSITORIES: biostudies-other