Transcription profiling of human MCF7 breast cancer cells MCF-7 two hours post incubation with heregulin (HRG)) vs. controls to detect HRG regulated genes

ABSTRACT: Heregulin beta-1 (HRG) is an extracellular ligand that activates mitogen-activated protein kinase (MAPK) and phosphatidylinositol-3-OH kinase (PI3K)/Akt signaling pathways through ErbB receptors. MAPK and Akt have been shown to phosphorylate the estrogen receptor (ER) at Ser-118 and Ser-167, respectively, thereby mimicking the effects of estrogenic activity such as estrogen responsive element (ERE)-dependent transcription. In the current study, integrative analysis was performed using two tiling array platforms, comprising histone H3 lysine 9 (H3K9) acetylation and RNA mapping, together with array comparative genomic hybridization (CGH) analysis in an effort to identify HRG-regulated genes in ER-positive MCF-7 breast cancer cells. Through application of various threshold settings, 333 (32

ORGANISM(S): Homo sapiens

SUBMITTER: Hatakeyama Mariko 

PROVIDER: S-ECPF-GEOD-8471 | biostudies-other |

REPOSITORIES: biostudies-other