Transcription profiling of human melanoma-endothelial cells co-cultured vs controls to investigate cellular communication

ABSTRACT: Average life expectancy for patients with metastatic melanoma is less than 6 months, and only a handful of treatment options are available. If the disease can be stopped before it spreads to other organs, life expectancy is greatly increased. The goal of this project is to identify possible regulators of melanoma metastasis by determining genes whose expression is modulated when the cells are grown in contact with endothelial cells. Identification of genes involved in this cell-cell communication could have therapeutic implications. Experiment Overall Design: Using a lentiviral system, melanoma cell line 1205Lu was transduced with GFP, and HUVEC (human umbillical vein endothelial cells) cells at passage 2 were transduced with RFP. These labeled cells were plated at near confluency either

ORGANISM(S): Homo sapiens

SUBMITTER: Keefe Megan Jean 

PROVIDER: S-ECPF-GEOD-8699 | biostudies-other |

REPOSITORIES: biostudies-other