Transcription profiling of normal breast epithelium of breast cancer patients reveals gene expression abnormalities

ABSTRACT: Normal-appearing epithelium of cancer patients can harbor occult genetic abnormalities. Data comprehensively comparing gene expression between histologically normal breast epithelium of breast cancer patients and cancer-free controls are limited. The present study compares global gene expression between these groups. We performed microarrays using RNA from microdissected histologically normal terminal ductal-lobular units (TDLU) from 2 groups: (i) cancer normal (CN) (TDLUs adjacent to untreated ER1 breast cancers (n = 14)) and (ii) reduction mammoplasty (RM) (TDLUs of age-matched women without breast disease (n = 15)). Cyber-T identi?ed differentially expressed genes. Quantitative RT-PCR (qRT-PCR), immunohistochemistry (IHC), and comparison to independent microarray data including 6 carcin

ORGANISM(S): Homo sapiens

SUBMITTER: Tripathi A 

PROVIDER: S-ECPF-GEOD-9574 | biostudies-other | 2008 Apr

REPOSITORIES: biostudies-other