Transcription profiling in breast cancer cell lines after Ctet treatment

ABSTRACT: Cyclic indole-3-carbinol (I3C) tetrameric derivative (CTet) is an anticancer molecule that has been shown to exert an antiproliferative activity in both MCF-7 and MDA-MB-231 breast cancer cell lines. To characterize the molecular mechanisms leading to the inhibition of the cell proliferation, gene expression analyses were conducted. MCF-7 and MDA-MB-231 breast cancer cells were plated in 6-wells culture plates at density of 150,000 cells/well and cultured overnight. Cellular treatments were conducted at 6uM and 12 uM concentration of CTet, or vehicle control, for 24 h. Cell survival was evaluated by trypan blue dye exclusion assay and, after washing in phosphate buffered saline (PBS), the cells were pelletted by centrifugation and stored at -20°C with 300µl of RNA-later solution (Sigma-ald

ORGANISM(S): Homo sapiens

SUBMITTER: Galluzzi L 

PROVIDER: S-ECPF-MEXP-2989 | biostudies-other | 2012

REPOSITORIES: biostudies-other