Transcription profiling by array of human SRC-2-depleted MCF-7 human breast cancer cells and MCF-7 cells exposed to PKA activating agents to identify genes that may be regulated through PKA-induced downregulation of SRC-2

ABSTRACT: We have characterized the global program of transcription in SRC-2-depleted MCF-7 breast cancer cells using short-hairpin RNA technology, and in MCF-7 cells exposed to PKA activating agents. In order to identify genes that may be regulated through PKA-induced downregulation of SRC-2, overlapping transcriptional targets in response to the respective treatments were characterized. MCF-7 human breast cancer cells transduced with shRNA targeting SRC-2 (SRC-2 shRNA) or infected with control shRNA vector (Ctr shRNA) were seeded in 9,2 cm petri dishes, 2,0 x106 cells in each dishes, in DMEM supplemented with 5% charcoaled stripped FBS and 10 nM 17b-estradiol. After 48 hours a selection of the Ctr shRNA cells were treated with cAMP analog (8-CPT-cAMP, 150 µM) and cAMP elevating agents (Forskolin

ORGANISM(S): Homo sapiens

SUBMITTER: Helland Thomas 

PROVIDER: S-ECPF-MTAB-1662 | biostudies-other |

REPOSITORIES: biostudies-other