Transcription profiling by array of human lung tumor tissue to determine the impact of collection and storage

ABSTRACT: We set out to investigate systematic changes in gene expression that may occur between surgical resection, routine processing in the histopathology department, frozen storage, and long term archiving in FFPE blocks. Lung carcinoma tissue samples were obtained from six patients during tumour resection surgery at the Royal Brompton Hospital, London.. When possible for each patient tissue samples were obtained at five collection points: viable tissue at the time of chest opening (T1a(CO)); immediately after resection (T1b(LR)); after transfer to histopathology (T2a(HP)); after transfer to histopathology and snap freezing (T2b(HP.SF)); and after transfer to histopathology and a week after being snap frozen and archived (T2c(HP.SFA)). FFPE samples of each patient’s tumour was obtained from hist

ORGANISM(S): Homo sapiens

SUBMITTER: Freidin MB 

PROVIDER: S-ECPF-MTAB-581 | biostudies-other | 2012 Mar-Apr

REPOSITORIES: biostudies-other