Transcription profiling of human primary breast tumors and matched short-term epithelial cultures

ABSTRACT: Background To identify the spectrum of malignant attributes maintained outside the host environment, we have compared global gene expression in primary breast tumors and matched short-term epithelial cultures. Results In contrast to immortal cell lines, a characteristic 'limited proliferation' phenotype was observed, which included over expressed genes associated with the TGFbeta signal transduction pathway, such as SPARC, LOXL1, RUNX1, and DAPK1. Underlying this profile was the conspicuous absence of hTERT expression and telomerase activity, a significant increase in TGFbeta receptor2, its cognate ligand, and the CDK inhibitor, p21CIP1/WAF1. Concurrently, tumor tissue and primary cultures displayed low transcript levels of proliferation-related genes, such as, TOP2A, ANKT, RAD51, UBE2C

ORGANISM(S): Homo sapiens

SUBMITTER: Dairkee SH 

PROVIDER: S-ECPF-SMDB-2888 | biostudies-other | 2004 Jul

REPOSITORIES: biostudies-other