Transcription profiling of immortalised human mammary luminal epithelial cells and variants expressing a moderate and high level of ERBB2

ABSTRACT: To investigate changes in gene expression associated with ERBB2, expression profiling of immortalised human mammary luminal epithelial cells and variants expressing a moderate and high level of ERBB2 has been carried out using cDNA microarrays corresponding to approximately 6,000 unique genes/ESTs. Sixty-three (63) significantly up- or down-regulated (>2.0 fold) genes were identified and further validated by RT-PCR and Northern blot analysis as well as microarray comparisons with a spontaneously ERBB2- overexpressing breast cancer cell line and ERBB2-positive primary breast tumours. Proteins predicted to be associated ERBB2 overexpression in breast cancers were analysed together with their clinical relevance by antibody screening using a tissue array. Differentially regulated genes include

ORGANISM(S): Homo sapiens

SUBMITTER: Dexter Tim 

PROVIDER: S-ECPF-SNGR-8 | biostudies-other |

REPOSITORIES: biostudies-other