Transcription profiling of MDA-MB-231 mammary cancer cells conditionally expressing progesterone receptor isoforms PRA and PRB

ABSTRACT: The progesterone receptor isoforms PRA and PRB are implicated in breast cancer development and metastasis. Expression ratio PRA/PRB is imbalanced in such cancers under the influence of MAPK-dependent extracellular stimuli that strongly impact progesterone-responsive transcriptional regulation. In order to determine the isoform-specific regulated genes in a metastatic cancer cell, we established the iPRAB cell line derived from MDA-MB-231 mammary cancer cells (ER-, PR-). This cell line conditionally expressed PRA and/or PRB under the control of RSL1 and doxycycline (Dox) non-steroid ligands, respectively, in a dose-dependent manner. The bi-inducible expression system was generated using a combination of the Rheoswitch (NE Biolabs) and T-Rex (Invitrogen) vector systems as described in the ar

ORGANISM(S): Homo sapiens

SUBMITTER: Loosfelt Hugues 

PROVIDER: S-ECPF-TABM-1215 | biostudies-other |

REPOSITORIES: biostudies-other