The cross-linking of tyrosine residues in apo-ovotransferrin by treatment with periodate anions.

ABSTRACT: The iron-binding ability of apotransferrins is rapidly abolished in the reaction with periodate anions, which destroys 4 mol of tyrosine per mol of protein. Treatment of ovotransferrin with cyanogen bromide and tryptic digestion of the glycopeptide fragment demonstrated the existence of an intramolecular cross-link in the C-terminal domain of the oxidized protein. The cross-linked residues were identified as Tyr-421 and Tyr-524 and the product is similar in structure to 3,3'-dityrosine.

SUBMITTER: Hsuan JJ 

PROVIDER: S-EPMC1148431 | biostudies-other | 1987 Oct

REPOSITORIES: biostudies-other