Adenylate cyclase activity in a higher plant, alfalfa (Medicago sativa).

ABSTRACT: An adenylate cyclase activity in Medicago sativa L. (alfalfa) roots was partially characterized. The enzyme activity remains in the supernatant fluid after centrifugation at 105,000 g and shows in crude extracts an apparent Mr of about 84,000. The enzyme is active with Mg2+ and Ca2+ as bivalent cations, and is inhibited by EGTA and by chlorpromazine. Calmodulin from bovine brain or spinach leaves activates this adenylate cyclase.

SUBMITTER: Carricarte VC 

PROVIDER: S-EPMC1148778 | biostudies-other | 1988 Feb

REPOSITORIES: biostudies-other