Development of a radioimmunoassay for rat liver metallothionein-I and its application to the analysis of rat plasma and kidneys.

ABSTRACT: A sensitive radioimmunoassay for rat liver metallothionein-I has been developed using avid and high-titre antibodies obtained from sheep that were immunized with a conjugate of metallothionein and rabbit immunoglobulin G. The assay was specific for metallothionein-I, and did not depend on the particular metal bound to the protein. There was no significant cross-reaction with rat liver metallothionein-II. The use of the assay to measure metallothionein concentrations in rat plasma and kidneys is described.

SUBMITTER: Mehra RK 

PROVIDER: S-EPMC1152148 | biostudies-other | 1983 Aug

REPOSITORIES: biostudies-other