Endocytosis of liposomes bound to cell surface proteins measured by flow cytofluorometry.
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ABSTRACT: A new technique for the quantification of cellular receptor-mediated endocytosis has been developed based on the analysis by flow cytometry of ligand-bearing liposomes containing the fluorochrome carboxyfluorescein. Carboxyfluorescein encapsulated at high concentrations in protein A-bearing liposomes is self-quenched. Binding and internalization of such liposomes by cells via antibodies directed towards membrane surface determinants results in the release of the liposome-encapsulated carboxyfluorescein into the cytoplasm causing an increase in cell-associated fluorescence. This increase can be quantified on a flow cytofluorometer.
SUBMITTER: Truneh A
PROVIDER: S-EPMC1152225 | biostudies-other | 1983 Jul
REPOSITORIES: biostudies-other
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