The SalGI restriction endonuclease. Purification and properties.

ABSTRACT: The type II restriction endonuclease SalGI has been purified to near homogeneity. At least 80% of the protein remaining after the final stage of the preparation is SalGI restriction endonuclease; no contaminating nucleases remain detectable. The principal form of the protein under both native and denaturing conditions is a monomer of M(r) about 29000. The optimal conditions for both enzyme stability and enzyme activity have been determined.

SUBMITTER: Maxwell A 

PROVIDER: S-EPMC1158195 | biostudies-other | 1982 Apr

REPOSITORIES: biostudies-other