Fractionation of membrane proteins on phenylboronic acid-agarose.

ABSTRACT: Detergent-solubilized plasma-membrane proteins from lymphocytes of patients with chronic lymphocytic leukaemia were applied to phenylboronic acid-agarose. Some of the polypeptides were bound specifically and subsequently eluted with sorbitol. Immunoprecipitation analyses showed that no surface immunoglobulin M was bound, but that most of the histocompatibility antigens HLA-A, HLA-B, HLA-C and HLA-DR were.

SUBMITTER: Williams GT 

PROVIDER: S-EPMC1158459 | biostudies-other | 1982 Jul

REPOSITORIES: biostudies-other