Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Calf rennet lysozyme.

ABSTRACT: A glycosidase displaying endo-N-acetylmuramoylhydrolase specificity (EC 3.2.1.17) was isolated from calf rennet. This lysozyme was also present in abomasal secretions from calf and adult cattle. Multiple molecular forms revealed by electrofocusing might be artefacts. The main enzyme form had Mr approx. 15 000, pH optimum 5.0, pI7.5, and a remarkable conformation stability. Competitive inhibition was observed with both N-acetylglucosamine and N-acetylmuramic acid, with apparent Ki values of 29 mM and 2.4 mM respectively. The isolated enzyme also displayed significant chitinase activity.

SUBMITTER: Pahud JJ

PROVIDER: S-EPMC1163696 | biostudies-other | 1982 Mar

REPOSITORIES: biostudies-other

ACCESS DATA

Json Xml

Similar Datasets

Calf

Project description:Assessing Faecalibacterium prausnitzii as a Psychobiotic in Pre-weaning Dairy Calves

| PRJNA1085263 | ENA

Calf

Project description:Early methanogen colonizers in preweaned dairy calves

| PRJNA1046510 | ENA

Bacillus subtilis Δ7ECF Pxyl-sigV + xylose vs Δ7ECF Pxyl-sigV - xylose , 168 + lysozyme vs 168 - lysozyme

Project description:To define the ECF sigma sigV - regulated genes during log growth phase in LB media under induction conditions for sigV The seven extracytoplasmic function (ECF) sigma (σ) factors of Bacillus subtilis are broadly implicated in resistance to antibiotics and other cell envelope stressors mediated, in part, by regulation of cell envelope synthesis and modification enzymes. We here define the regulon of σV as including at least 20 operons many of which are also regulated by σM, σX, or σW. The σV regulon is strongly and specifically induced by lysozyme and this induction is key to the intrinsic resistance of B. subtilis to lysozyme. Strains with null mutations in either sigV or in all seven ECF σ factor genes (Δ7ECF) have essentially equal increases in sensitivity to lysozyme. Induction of σV in the Δ7ECF background restores lysozyme resistance, whereas induction of σM, σX or σW does not. Lysozyme resistance results from the ability of σV to activate the transcription of two operons: the autoregulated sigV-rsiV-oatA-yrhK operon and dltABCDE. Genetic analyses reveal that oatA and dlt are largely redundant with respect to lysozyme sensitivity: single mutants are not affected in lysozyme sensitivity whereas a double oatA dltA mutant is as sensitive as a sigV null strain. Moreover, the triple sigV oatA dltA mutant is no more sensitive than the oatA dltA double mutant, indicating that there are no other σV-dependent genes necessary for lysozyme resistance. Thus, σV confers lysozyme resistance by activation of two cell wall modification pathways: O-acetylation of peptidoglycan catalyzed by OatA and D-alanylation of teichoic acids by DltABCDE.

2011-08-23 | GSE31563 | GEO

Calf feces

Project description:Calf feces Raw sequence reads

| PRJNA506828 | ENA

Calf faeces

Project description:Calf faeces Raw sequence reads

| PRJNA1079492 | ENA

dairy calf

Project description:dairy calf Targeted loci

| PRJNA380152 | ENA

Calf Metagenomics

Project description:Comparison of the fecal bacterial communities in diarrheic and healthy dairy calves from multiple farms in Southeastern PA

| PRJNA635612 | ENA

dairy calf

Project description:16S rRNA amplicon sequencing was used to characterize mucosa- and digesta-associated microbiota in the hindgut (cecum, colon and rectum) of pre-weaned dairy calves Targeted loci

| PRJNA379626 | ENA

Simmental calf

Project description:Simmental calf Raw sequence reads

| PRJNA1071495 | ENA

Calf metagenome

Project description:Maternal methionine effects on calf microbiome

| PRJNA508512 | ENA