Adenosine triphosphate-evoked catecholamine release in chromatin granules. Osmotic lysis as a consequence of proton translocation.

ABSTRACT: Chromaffin granules suspended in C1-containing media release catecholamine and protein when ATP is added. This phenomenon is inhibited in hyperosmotic media and in the presence of uncouplers of oxidative phosphorylation. Release requires a permeant anion in the medium, but is independent of the cation. The release process appears to be driven by an inwardly directed proton-translocating adenosine triphosphatase. The resulting proton-anion influx causes osmotic lysis of the chromaffin granules.

SUBMITTER: Casey RP 

PROVIDER: S-EPMC1164013 | biostudies-other | 1976 Sep

REPOSITORIES: biostudies-other