Removal of a bound ligand from a macromolecule by gel filtration.

ABSTRACT: If a solution of macromolecule of concentration c and volume a, initially saturated with a ligand of dissociation constant K, is gel filtered through a volume v of solution initially free of ligand, the saturation y with which it emerges is given by the equation y-lny-1=Kv/ca.

SUBMITTER: Dixion HB 

PROVIDER: S-EPMC1164049 | biostudies-other | 1976 Oct

REPOSITORIES: biostudies-other