Statistical considerations in the estimation of enzyme kinetic parameters by the direct linear plot andother methods.

ABSTRACT: The statistical implications of the direct linear plot for enzyme kinetic data, described in the preceding paper (Eisenthal & Cornish-Bowden, 1974), are discussed for the case of the Michaelis-Menten equation. The plot is shown to lead directly to non-parametric confidence limits for the kinetic parameters, V and K(m), which depend on far less sweeping assumptions about the nature of experimental error than those implicit in the method of least squares. Median estimates of V and K(m) can also be defined, which are shown to be more robust than the least-squares estimates in a wide variety of experimental situations.

SUBMITTER: Cornish-Bowden A 

PROVIDER: S-EPMC1166336 | biostudies-other | 1974 Jun

REPOSITORIES: biostudies-other