Peroxidatic oxidation of tyrosine to melanin in supernatant of crude mouse melanoma homogenates.

ABSTRACT: Peroxide-dependent enzymic oxidation of tyrosine to dopachrome and melanin was demonstrated in cell-free melanoma homogenates. Histochemical methods for distinguishing peroxidase activity from aerobic dopa (3,4-dihydroxyphenylalanine) oxidase activity are not reliable with cell-free preparations. Therefore the presence of peroxidase activity in such preparations precludes assay of cresolase activity of mammalian ;tyrosinase'.

SUBMITTER: Patel RP 

PROVIDER: S-EPMC1168298 | biostudies-other | 1974 Aug

REPOSITORIES: biostudies-other