Unknown

Dataset Information

0

The effect of acid proteinase inhibitors on chicken pepsin.


ABSTRACT: 1. The activity of chicken pepsin was partially inhibited by dimethyl-(2-hydroxy-5-nitrobenzyl)sulphonium bromide, but was unaffected by p-bromophenacyl bromide. 2. In the presence of Cu2+, diazoacetylnorleucine methyl ester completely inactivated chicken pepsin with the incorporation of 1 mol/mol. The mechanism of the reaction was similar to that with pig pepsin. 3. Chicken pepsin was completely inactivated by 2-diazo-4-bromoacetophenone in the presence of Cu2+. 4. Chicken pepsin was almost completely inactivated by 1,2-epoxy-3-(p-nitrophenoxy)propane at 25 degrees C, 3-4mol of inhibitor/mol being incorporated. The reaction at 10 degrees C was investigated briefly. 5. Calf chymosin was inactivated by 1,2-epoxy-3-(p-nitrophenoxy)propane at 10 degrees C, the incorporation of 1 mol/mol being required for complete inhibition. 6. The characteristics of the reactions of chicken pepsin with the above compounds were compared with those of other acid proteinases.

SUBMITTER: Llewellin JM 

PROVIDER: S-EPMC1172362 | biostudies-other | 1975 Nov

REPOSITORIES: biostudies-other

Similar Datasets

| S-EPMC2930701 | biostudies-literature
| S-EPMC6093747 | biostudies-literature
| S-EPMC6760148 | biostudies-literature
| S-EPMC7119134 | biostudies-literature
| S-EPMC3810942 | biostudies-literature
| S-EPMC8358037 | biostudies-literature
2009-10-01 | GSE17523 | GEO
| S-EPMC1172428 | biostudies-other
| S-EPMC3003809 | biostudies-literature
| S-EPMC5312868 | biostudies-literature