Purification of ribonuclease T 1 by diethylaminoethylcellulose chromatography.

ABSTRACT: A procedure is described for isolating the enzyme ribonuclease T(1) from Takadiastase, an extract of the mould Aspergillus oryzae. It involves an initial concentration of the enzyme by adsorption on DEAE-cellulose followed by gradient elution. Later the enzyme is chromatographed on the same adsorbent with an eluent of constant composition. Yields of 350-380mg of ribonuclease T(1) from 500g of Takadiastase were obtained.

SUBMITTER: Fields R 

PROVIDER: S-EPMC1176633 | biostudies-other | 1971 Feb

REPOSITORIES: biostudies-other