The number of catalytic sites in acetylcholinesterase.

ABSTRACT: By using two methods of titration, the number of active sites in acetylcholinesterase was determined. Either stepwise inhibition of the enzyme by an irreversible inhibitor, namely di-isopropyl phosphorofluoridate, or direct measurement of the concentration of active sites by titration with o-nitrophenyl dimethylcarbamate yielded an equivalent weight of approx. 130000 for an active site in acetylcholinesterase. This indicates two sites per molecule, since the native enzyme has a molecular weight of 260000.

SUBMITTER: Leuzinger W 

PROVIDER: S-EPMC1176916 | biostudies-other | 1971 Jun

REPOSITORIES: biostudies-other