Characterization of Saccharomyces cerevisiae deficient in expression of phospholipase D.

ABSTRACT: A gene encoding phospholipase D (PLD) in Saccharomyces cerevisiae was identified. The 195 kDa product of PLD1 has 24% overall sequence identity with a plant PLD. Expression of yeast PLD activity was eliminated by one-step gene disruption. Yeast haploids lacking PLD activity were deficient in growth on non-fermentable carbon sources. Diploids lacking expression of PLD1 were unable to sporulate.

SUBMITTER: Ella KM 

PROVIDER: S-EPMC1217018 | biostudies-other | 1996 Feb

REPOSITORIES: biostudies-other