The study of cell-death proteins in the outer mitochondrial membrane by chemical cross-linking.

ABSTRACT: Chemical cross-linking was used to study the interactions of the anti-cell-death protein Bcl2 with other proteins in the outer mitochondrial membrane. Cross-linking of mitochondrial surface proteins produced a large Bcl2-containing complex (>200 kDa), and a Bcl2-derived peptide was shown to cross-link specifically with a mitochondrial protein identified by immunoblotting as Raf-1 kinase.

SUBMITTER: Ali ST 

PROVIDER: S-EPMC1218563 | biostudies-other | 1997 Jul

REPOSITORIES: biostudies-other