Unknown

Dataset Information

0

Differential regulation of nitric oxide synthase mRNA expression by lipopolysaccharide and pro-inflammatory cytokines in fetal hepatocytes treated with cycloheximide.


ABSTRACT: The effect of cycloheximide (CHX) on the mRNA expression of the cytokine-inducible, calcium-independent nitric oxide synthase (iNOS) was investigated in fetal hepatocytes stimulated with lipopolysaccharide (LPS) or pro-inflammatory cytokines. In the presence of CHX the LPS-dependent iNOS mRNA levels were reduced, whereas the response to pro-inflammatory cytokines was enhanced. Because iNOS transcription is highly dependent on the activation of nuclear factor kappaB (NF-kappaB), this factor was evaluated by electrophoretic mobility shift assays, and a close correlation between NF-kappaB activity and iNOS mRNA levels was observed. CHX itself potentiated the degradation of the IkappaB alpha and IkappaB beta inhibitory subunits (IkappaB is inhibitory kappaB) of the NF-kappaB complex, and therefore the loss of LPS-dependent iNOS mRNA expression cannot be attributed to a blockage in the activation of NF-kappaB. These results suggest the existence of a CHX-sensitive pathway in the expression of iNOS mediated by LPS, a mechanism that is not involved in the response to pro-inflammatory cytokines.

SUBMITTER: Casado M 

PROVIDER: S-EPMC1218862 | biostudies-other | 1997 Nov

REPOSITORIES: biostudies-other

Similar Datasets

| S-EPMC9010940 | biostudies-literature
| S-EPMC9740548 | biostudies-literature
| S-EPMC8475549 | biostudies-literature
| S-EPMC5620245 | biostudies-literature
| S-EPMC4430446 | biostudies-literature
| S-EPMC7735382 | biostudies-literature
| S-EPMC4229106 | biostudies-literature
| S-EPMC9285385 | biostudies-literature
| S-EPMC5376205 | biostudies-literature
| S-EPMC3217123 | biostudies-literature