Unknown

Dataset Information

0

Identification of a cAMP response element within the glucose- 6-phosphatase hydrolytic subunit gene promoter which is involved in the transcriptional regulation by cAMP and glucocorticoids in H4IIE hepatoma cells.


ABSTRACT: The expression of a luciferase reporter gene under the control of the human glucose 6-phosphatase gene promoter was stimulated by both dexamethasone and dibutyryl cAMP in H4IIE hepatoma cells. A cis-active element located between nucleotides -161 and -152 in the glucose 6-phosphatase gene promoter was identified and found to be necessary for both basal reporter-gene expression and induction of expression by both dibutyryl cAMP and dexamethasone. Nucleotides -161 to -152 were functionally replaced by the consensus sequence for a cAMP response element. An antibody against the cAMP response element-binding protein caused a supershift in gel-electrophoretic-mobility-shift assays using an oligonucleotide probe representing the glucose 6-phosphatase gene promoter from nucleotides -161 to -152. These results strongly indicate that in H4IIE cells the glucose 6-phosphatase gene-promoter sequence from -161 to -152 is a cAMP response element which is important for the regulation of transcription of the glucose 6-phosphatase gene by both cAMP and glucocorticoids.

SUBMITTER: Schmoll D 

PROVIDER: S-EPMC1220073 | biostudies-other | 1999 Mar

REPOSITORIES: biostudies-other

Similar Datasets

| S-EPMC2676107 | biostudies-literature
| S-EPMC2966426 | biostudies-literature
| S-EPMC3840356 | biostudies-literature
| S-EPMC5074532 | biostudies-literature
| S-EPMC3089798 | biostudies-literature
| S-EPMC5614554 | biostudies-literature
| S-EPMC1223301 | biostudies-other
| S-EPMC6499384 | biostudies-literature
| S-EPMC2963359 | biostudies-literature
| S-EPMC6109926 | biostudies-literature