Ontology highlight
ABSTRACT:
SUBMITTER: Belk JP
PROVIDER: S-EPMC1369829 | biostudies-other | 1999 Aug
REPOSITORIES: biostudies-other
Belk J P JP He F F Jacobson A A
RNA (New York, N.Y.) 19990801 8
The yeast NMD3 gene was identified in a two-hybrid screen using the nonsense-mediated mRNA decay factor, Upf1p, as bait. NMD3 was shown to encode an essential, highly conserved protein that associated principally with free 60S ribosomal subunits. Overexpression of a truncated form of Nmd3p, lacking 100 C-terminal amino acids and most of its Upf1p-interacting domain, had dominant-negative effects on both cell growth and protein synthesis and promoted the formation of polyribosome half-mers. These ...[more]