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DNA Extraction and PCR Methods for the Detection of Listeria monocytogenes in Cold-Smoked Salmon.

ABSTRACT: Protocols for the specific detection of Listeria monocytogenes in cold-smoked salmon were developed. PCR was used as the method of detection. Inhibitors of PCR present in the food samples were removed by ether extraction or column purification, or their effect was overcome by the use of Tween 20 as an enhancer. These protocols are many times more rapid than conventional detection methodologies and also have the potential for automation.

SUBMITTER: Simon MC

PROVIDER: S-EPMC1388797 | biostudies-other | 1996 Mar

REPOSITORIES: biostudies-other

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DNA Extraction and PCR Methods for the Detection of Listeria monocytogenes in Cold-Smoked Salmon.

Simon M C MC Gray D I DI Cook N N

Applied and environmental microbiology 19960301 3

Protocols for the specific detection of Listeria monocytogenes in cold-smoked salmon were developed. PCR was used as the method of detection. Inhibitors of PCR present in the food samples were removed by ether extraction or column purification, or their effect was overcome by the use of Tween 20 as an enhancer. These protocols are many times more rapid than conventional detection methodologies and also have the potential for automation. ...[more]

PMID: 16535272

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