Isolation of developmentally regulated genes by differential display screening of cDNA libraries.
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ABSTRACT:
SUBMITTER: Gupta R
PROVIDER: S-EPMC147855 | biostudies-other | 1998 Oct
REPOSITORIES: biostudies-other
Publications
Isolation of developmentally regulated genes by differential display screening of cDNA libraries.
Gupta R R Thomas P P Beddington R S RS Rigby P W PW
Nucleic acids research 19981001 19
mRNA differential display RT-PCR has been extensively used for the isolation of genes differentially expressed between RNA populations. We have assessed its utility for the identification of developmentally regulated genes in plasmid cDNA libraries derived from individual tissues dissected from early mouse embryos. Using plasmid Southern blot hybridisation as a secondary screen, we are able to identify such genes and show by whole-mount in situ hybridisation that their expression pattern is that ...[more]
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