Unknown

Dataset Information

0

Pyrimidine biosynthesis genes (pyrE and pyrF) of an extreme thermophile, Thermus thermophilus.

ABSTRACT: We have isolated uracil auxotrophic mutants of an extreme thermophile, Thermus thermophilus. A part of the pyrimidine biosynthetic operon including genes for orotate phosphoribosyltransferase (pyrE) and for orotidine-5'-monophosphate decarboxylase (pyrF) was cloned and sequenced. The pyrE gene can be a bidirectional marker for the gene manipulation system of the thermophile.

SUBMITTER: Yamagishi A 

PROVIDER: S-EPMC167999 | biostudies-other | 1996 Jun

REPOSITORIES: biostudies-other

Json Xml
altmetric image

Publications

Pyrimidine biosynthesis genes (pyrE and pyrF) of an extreme thermophile, Thermus thermophilus.

Yamagishi A A   Tanimoto T T   Suzuki T T   Oshima T T  

Applied and environmental microbiology 19960601 6

We have isolated uracil auxotrophic mutants of an extreme thermophile, Thermus thermophilus. A part of the pyrimidine biosynthetic operon including genes for orotate phosphoribosyltransferase (pyrE) and for orotidine-5'-monophosphate decarboxylase (pyrF) was cloned and sequenced. The pyrE gene can be a bidirectional marker for the gene manipulation system of the thermophile. ...[more]

Similar Datasets

Unknown Allosteric regulation in phosphofructokinase from the extreme thermophile Thermus thermophilus.
| S-EPMC3982590 | biostudies-literature
Unknown Molecular cloning of the pyrE gene from the extreme thermophile Thermus flavus.
| S-EPMC177211 | biostudies-other
Unknown Efficient genome editing of an extreme thermophile, Thermus thermophilus, using a thermostable Cas9 variant.
| S-EPMC8100143 | biostudies-literature
Unknown Genomic and proteomic characterization of the large Myoviridae bacteriophage ?TMA of the extreme thermophile Thermus thermophilus.
| S-EPMC3225780 | biostudies-literature
Unknown Efficient trans-cleavage by the Schistosoma mansoni SMalpha1 hammerhead ribozyme in the extreme thermophile Thermus thermophilus.
| S-EPMC101841 | biostudies-literature
Unknown Transposition of an insertion sequence, ISTth7, in the genome of the extreme thermophile Thermus thermophilus HB8.
| S-EPMC3643509 | biostudies-literature
Unknown Functional expression of a penicillin acylase from the extreme thermophile Thermus thermophilus HB27 in Escherichia coli.
| S-EPMC3461476 | biostudies-literature
Unknown Stabilization of nucleic acids by unusual polyamines produced by an extreme thermophile, Thermus thermophilus.
| S-EPMC1138949 | biostudies-other
Unknown Properties of the lysyl-tRNA synthetase gene and product from the extreme thermophile Thermus thermophilus.
| S-EPMC205411 | biostudies-other
Unknown Structure and expression of a pyrimidine gene cluster from the extreme thermophile Thermus strain ZO5.
| S-EPMC179137 | biostudies-other