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The alanine racemase gene is essential for growth of Lactobacillus plantarum.

ABSTRACT: The Lactobacillus plantarum alr gene encoding alanine racemase was cloned by complementation of an Escherichia coli Alr- DadX- double mutant strain. Knockout of the alr gene abolished all measurable alanine racemase activity, and the mutant was shown to be strictly dependent on D-alanine for growth.

SUBMITTER: Hols P 

PROVIDER: S-EPMC179184 | biostudies-other | 1997 Jun

REPOSITORIES: biostudies-other

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The alanine racemase gene is essential for growth of Lactobacillus plantarum.

Hols P P   Defrenne C C   Ferain T T   Derzelle S S   Delplace B B   Delcour J J  

Journal of bacteriology 19970601 11

The Lactobacillus plantarum alr gene encoding alanine racemase was cloned by complementation of an Escherichia coli Alr- DadX- double mutant strain. Knockout of the alr gene abolished all measurable alanine racemase activity, and the mutant was shown to be strictly dependent on D-alanine for growth. ...[more]

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