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Binding motifs predict major histocompatibility complex class II-restricted epitopes in the Sendai virus M protein.

ABSTRACT: Major histocompatibility complex (MHC) class I ligand motifs have been defined for a number of class I molecules and have been successfully used to identify class I-restricted cytotoxic T-cell epitopes. In contrast, the relative degeneracy of sequence motifs in naturally processed MHC class II ligands has suggested that they may be of more limited use. Here, we use a predicted I-Ab ligand motif to identify antigenic peptides in the Sendai virus Enders strain matrix (M) protein. The entire coding sequence of the M protein was derived, and seven peptide sequences that contained the predicted I-Ab motif were identified. Analysis of I-Ab-restricted M-specific T-cell hybridomas for reactivity to these synthetic peptides identified two distinct epitopes. These data demonstrate that MHC class II motifs can be valuable in predicting T-cell epitopes.

SUBMITTER: Cole GA 

PROVIDER: S-EPMC189753 | biostudies-other | 1995 Dec

REPOSITORIES: biostudies-other

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Binding motifs predict major histocompatibility complex class II-restricted epitopes in the Sendai virus M protein.

Cole G A GA   Tao T T   Hogg T L TL   Ryan K W KW   Woodland D L DL  

Journal of virology 19951201 12

Major histocompatibility complex (MHC) class I ligand motifs have been defined for a number of class I molecules and have been successfully used to identify class I-restricted cytotoxic T-cell epitopes. In contrast, the relative degeneracy of sequence motifs in naturally processed MHC class II ligands has suggested that they may be of more limited use. Here, we use a predicted I-Ab ligand motif to identify antigenic peptides in the Sendai virus Enders strain matrix (M) protein. The entire coding  ...[more]