Dataset Information

Phylogenetic analysis of the genus Borrelia: a comparison of North American and European isolates of Borrelia burgdorferi.

ABSTRACT: We have sequenced the 16S rRNA molecules from four species of Borrelia and from six isolates of Borrelia burgdorferi via the reverse transcriptase primer extension method. The sequences were aligned and evolutionary relationships were determined, including the calculation of evolutionary distances and the construction of a phylogenetic tree. These analyses demonstrate significant divergence among B. burgdorferi isolates, with the European isolates G1 and G2 residing most distant from the main cluster. Signature nucleotides which distinguish B. burgdorferi from all other members of this genus and which distinguish the European isolates G1 and G2 from the North American isolates B31, Sh-2-82, and 1352 were identified. Finally, Southern blot analyses were performed to compare the restriction patterns of the genes coding for rRNA and to relate our data to the grouping scheme of Postic et al. (D. Postic, C. Edlinger, C. Richaud, F. Grimont, J. Dufresne, P. Perolat, G. Baranton, and P. A. D. Grimont, Res. Microbiol. 141:465-475, 1990).

SUBMITTER: Marconi RT

PROVIDER: S-EPMC205701 | biostudies-other | 1992 Jan

REPOSITORIES: biostudies-other

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Phylogenetic analysis of the genus Borrelia: a comparison of North American and European isolates of Borrelia burgdorferi.

Marconi R T RT Garon C F CF

Journal of bacteriology 19920101 1

We have sequenced the 16S rRNA molecules from four species of Borrelia and from six isolates of Borrelia burgdorferi via the reverse transcriptase primer extension method. The sequences were aligned and evolutionary relationships were determined, including the calculation of evolutionary distances and the construction of a phylogenetic tree. These analyses demonstrate significant divergence among B. burgdorferi isolates, with the European isolates G1 and G2 residing most distant from the main cl ...[more]

PMID: 1370282

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Project description:BACKGROUND:North American Agalinis Raf. species represent a taxonomically challenging group and there have been extensive historical revisions at the species, section, and subsection levels of classification. The genus contains many rare species, including the federally listed endangered species Agalinis acuta. In addition to evaluating the degree to which historical classifications at the section and subsection levels are supported by molecular data sampled from 79 individuals representing 29 Agalinis species, we assessed the monophyly of 27 species by sampling multiple individuals representing different populations of those species. Twenty-one of these species are of conservation concern in at least some part of their range. RESULTS:Phylogenetic relationships estimated using maximum likelihood analyses of seven chloroplast DNA loci (aligned length = 11,076 base pairs (bp) and the nuclear ribosomal DNA ITS (internal transcribed spacer) locus (733 bp); indicated no support for the historically recognized sections except for Section Erectae. Our results suggest that North American members of the genus comprise six major lineages, however we were not able to resolve branching order among many of these lineages. Monophyly of 24 of the 29 sampled species was supported based on significant branch lengths of and high bootstrap support for subtending branches. However, there was no statistical support for the monophyly of A. acuta with respect to Agalinis tenella and Agalinis decemloba. Although most species were supported, deeper relationships among many species remain ambiguous. CONCLUSION:The North American Agalinis species sampled form a well supported, monophyletic group within the family Orobanchaceae relative to the outgroups sampled. Most hypotheses regarding section- and subsection-level relationships based on morphology were not supported and taxonomic revisions are warranted. Lack of support for monophyly of Agalinis acuta leaves the important question regarding its taxonomic status unanswered. Lack of resolution is potentially due to incomplete lineage sorting of ancestral polymorphisms among recently diverged species; however the gene regions examined did distinguish among almost all other species in the genus. Due to the important policy implications of this finding we are further evaluating the evolutionary distinctiveness of A. acuta using morphological data and loci with higher mutation rates.

Dataset Information

Phylogenetic analysis of the genus Borrelia: a comparison of North American and European isolates of Borrelia burgdorferi.

Publications

Phylogenetic analysis of the genus Borrelia: a comparison of North American and European isolates of Borrelia burgdorferi.

Similar Datasets

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