Ontology highlight
ABSTRACT:
SUBMITTER: Dange T
PROVIDER: S-EPMC2557044 | biostudies-other | 2008 Oct
REPOSITORIES: biostudies-other
The Journal of cell biology 20080929 1
All molecular traffic between nucleus and cytoplasm occurs via the nuclear pore complex (NPC) within the nuclear envelope. In this study we analyzed the interactions of the nuclear transport receptors kapalpha2, kapbeta1, kapbeta1DeltaN44, and kapbeta2, and the model transport substrate, BSA-NLS, with NPCs to determine binding sites and kinetics using single-molecule microscopy in living cells. Recombinant transport receptors and BSA-NLS were fluorescently labeled by AlexaFluor 488, and microinj ...[more]