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Identification of a New ss-1,4-endoglucanase Gene Expressed in the Esophageal Subventral Gland Cells of Heterodera glycines.

ABSTRACT: Secretory proteins encoded by parasitism genes expressed in the esophageal gland cells of plant-parasitic nematodes play key roles in nematode-plant interactions. A fourth ss-1,4-endoglucanase full-length cDNA (designated Hg-eng-4) was isolated from a Heterodera glycines esophageal gland-cell long-distance polymerase chain reaction cDNA library. The cDNA hybridized to genomic DNA of H. glycines in Southern blots. The Hg-eng-4 cDNA contained an open reading frame encoding 352 amino acids, with the first 18 amino acids being a putative secretion signal. Hg-ENG-4 contained a family 5 endoglucanase catalytic domain and a peptide linker of repeat amino acids, but no cellulose binding domain. In-situ hybridization analyses showed that transcripts of Hg-eng-4 accumulated specifically in the subventral gland cells of pre-parasitic and parasitic second-stage juveniles of H. glycines.

SUBMITTER: Gao B

PROVIDER: S-EPMC2620536 | biostudies-other | 2002 Mar

REPOSITORIES: biostudies-other

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Identification of a New ss-1,4-endoglucanase Gene Expressed in the Esophageal Subventral Gland Cells of Heterodera glycines.

Gao B B Allen R R Maier T T Davis E L EL Baum T J TJ Hussey R S RS

Journal of nematology 20020301 1

Secretory proteins encoded by parasitism genes expressed in the esophageal gland cells of plant-parasitic nematodes play key roles in nematode-plant interactions. A fourth ss-1,4-endoglucanase full-length cDNA (designated Hg-eng-4) was isolated from a Heterodera glycines esophageal gland-cell long-distance polymerase chain reaction cDNA library. The cDNA hybridized to genomic DNA of H. glycines in Southern blots. The Hg-eng-4 cDNA contained an open reading frame encoding 352 amino acids, with th ...[more]

PMID: 19265901

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Project description:The soybean cyst nematode (SCN), Heterodera glycines, causes economically significant damage to soybeans (Glycine max) in many parts of the world. The cysts of this nematode can remain quiescent in soils for many years as a reservoir of infection for future crops. To investigate bacterial communities associated with SCN cysts, cysts were obtained from eight SCN-infested farms in southern Ontario, Canada, and analyzed by culture-dependent and -independent means. Confocal laser scanning microscopy observations of cyst contents revealed a microbial flora located on the cyst exterior, within a polymer plug region and within the cyst. Microscopic counts using 5-(4,6-dichlorotriazine-2-yl)aminofluorescein staining and in situ hybridization (EUB 338) indicated that the cysts contained (2.6 +/- 0.5) x 10(5) bacteria (mean +/- standard deviation) with various cellular morphologies. Filamentous fungi were also observed. Live-dead staining indicated that the majority of cyst bacteria were viable. The probe Nile red also bound to the interior polymer, indicating that it is lipid rich in nature. Bacterial community profiles determined by denaturing gradient gel electrophoresis analysis were simple in composition. Bands shared by all eight samples included the actinobacterium genera Actinomadura and STREPTOMYCES: A collection of 290 bacteria were obtained by plating macerated surface-sterilized cysts onto nutrient broth yeast extract agar or on actinomycete medium. These were clustered into groups of siblings by repetitive extragenic palindromic PCR fingerprinting, and representative isolates were tentatively identified on the basis of 16S rRNA gene sequence. Thirty phylotypes were detected, with the collection dominated by Lysobacter and Variovorax spp. This study has revealed the cysts of this important plant pathogen to be rich in a variety of bacteria, some of which could presumably play a role in the ecology of SCN or have potential as biocontrol agents.

Dataset Information

Identification of a New ss-1,4-endoglucanase Gene Expressed in the Esophageal Subventral Gland Cells of Heterodera glycines.

Publications

Identification of a New ss-1,4-endoglucanase Gene Expressed in the Esophageal Subventral Gland Cells of Heterodera glycines.

Similar Datasets

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