Ontology highlight
ABSTRACT:
SUBMITTER: Tay YD
PROVIDER: S-EPMC2857021 | biostudies-other | 2010 Apr
REPOSITORIES: biostudies-other
The Journal of biological chemistry 20100222 15
Eukaryotic Holliday junction (HJ) resolvases have attracted much attention recently with the identification of at least three distinct proteins that can cleave model HJs in vitro. However, the specific DNA structure(s) that these proteins act upon in the cell is unknown. Here, we describe a system in budding yeast to directly and quantitatively monitor in vivo HJ resolution. We found that Yen1 acts redundantly with Mus81, but not Slx1, to resolve a model HJ in vivo. This functional overlap speci ...[more]