Dataset Information
Rational design and PCR-based synthesis of an artificial Schizophyllum commune xylanase gene.
Ontology highlight
ABSTRACT:
SUBMITTER: Graham RW
PROVIDER: S-EPMC311407 | biostudies-other | 1993 Oct
REPOSITORIES: biostudies-other
Publications
Rational design and PCR-based synthesis of an artificial Schizophyllum commune xylanase gene.
Nucleic acids research 19931001 21
A synthetic gene encoding the Schizophyllum commune xylanase XynA was constructed by a novel PCR-based procedure. Three long oligonucleotides were synthesized and used in combination with flanking PCR primers to generate a 607 base pair gene which contained 31 unique locations for restriction enzyme cleavage. The amino acid sequence was tailored for expression in Escherichia coli by using only those codons found in highly expressed E. coli genes. The availability of the gene will facilitate anal ...[more]
