Ontology highlight
ABSTRACT:
SUBMITTER: Barakat TS
PROVIDER: S-EPMC3185440 | biostudies-other | 2011 Oct
REPOSITORIES: biostudies-other
Nucleic acids research 20110707 18
The use of bacterial artificial chromosomes (BACs) provides a consistent and high targeting efficiency of homologous recombination in embryonic stem (ES) cells, facilitated by long stretches of sequence homology. Here, we introduce a BAC targeting method which employs restriction fragment length polymorphisms (RFLPs) in targeted polymorphic C57BL/6/Cast/Ei F1 mouse ES cell lines to identify properly targeted ES cell clones. We demonstrate that knockout alleles can be generated either by targetin ...[more]