Unknown

Dataset Information

0

Heterologous activation of the actinorhodin biosynthetic pathway in Streptomyces lividans.

ABSTRACT: A DNA fragment of Streptomyces fradiae is able to activate the antibiotic actinorhodin biosynthetic pathway when cloned in Streptomyces lividans. The activator DNA region has been sequenced and its transcription initiation and termination sites accurately mapped in vivo. This DNA encodes a 132 nucleotides long transcript which is apparently responsible for the actinorhodin production phenotype, possibly acting as an antisense RNA. The sequence of the activator gene revealed no homology with any other known Streptomyces coelicolor genes concerned with actinorhodin biosynthesis or its pleiotropic regulation.

SUBMITTER: Romero NM 

PROVIDER: S-EPMC336920 | biostudies-other | 1992 Jun

REPOSITORIES: biostudies-other

Json Xml
altmetric image

Publications

Heterologous activation of the actinorhodin biosynthetic pathway in Streptomyces lividans.

Romero N M NM   Parro V V   Malpartida F F   Mellado R P RP  

Nucleic acids research 19920601 11

A DNA fragment of Streptomyces fradiae is able to activate the antibiotic actinorhodin biosynthetic pathway when cloned in Streptomyces lividans. The activator DNA region has been sequenced and its transcription initiation and termination sites accurately mapped in vivo. This DNA encodes a 132 nucleotides long transcript which is apparently responsible for the actinorhodin production phenotype, possibly acting as an antisense RNA. The sequence of the activator gene revealed no homology with any  ...[more]

Similar Datasets

Unknown Transcriptomic and fluxomic changes in Streptomyces lividans producing heterologous protein.
| S-EPMC6302529 | biostudies-literature
Unknown Twin-arginine translocation pathway in Streptomyces lividans.
| S-EPMC95510 | biostudies-literature
Unknown Engineering of Streptomyces lividans for heterologous expression of secondary metabolite gene clusters.
| S-EPMC6950998 | biostudies-literature
Unknown Identification of the thiazolyl peptide GE37468 gene cluster from Streptomyces ATCC 55365 and heterologous expression in Streptomyces lividans.
| S-EPMC3156177 | biostudies-literature
Unknown Engineered Streptomyces lividans Strains for Optimal Identification and Expression of Cryptic Biosynthetic Gene Clusters.
| S-EPMC6295570 | biostudies-literature
Transcriptomics Agarase overproduction in Streptomyces lividans
2015-07-23 | GSE61447 | GEO
Transcriptomics The Streptomyces lividans lsp mutant
2014-05-22 | GSE30728 | GEO
Transcriptomics Alpha-amylase overproduction in Streptomyces lividans
2015-07-23 | GSE61448 | GEO
Unknown Engineered Streptomyces avermitilis host for heterologous expression of biosynthetic gene cluster for secondary metabolites.
| S-EPMC3932656 | biostudies-literature
Unknown Direct cloning and heterologous expression of the salinomycin biosynthetic gene cluster from Streptomyces albus DSM41398 in Streptomyces coelicolor A3(2).
| S-EPMC4602208 | biostudies-literature