RNA-guided genome editing a la carte.

ABSTRACT: Two recent papers in Science illustrate how the prokaryotic CRISPR-Cas immune system machinery, which typically targets invasive genetic elements such as viruses and plasmids, can be converted into a sophisticated molecular tool for next-generation human genome editing. The versatile Cas9 RNA-guided endonuclease can be readily reprogrammed using customizable small RNAs for sequence-specific single- or double-stranded DNA cleavage.

SUBMITTER: Horvath P 

PROVIDER: S-EPMC3674385 | biostudies-other | 2013 Jun

REPOSITORIES: biostudies-other